Conductive Hairy Particles With Homogeneous and Janus Design as Carrier Materials for the Efficient Immobilization of Unspecific Peroxygenases

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Karich, Alexander ; Cai, Hongtao ; Linhardt, Anne ; Antony, Anila ; Liers, Christiane ; Ullrich, René ; Schwaderer, Fabian ; Kalmbach, Johannes ; Scheibner, Katrin ; Hofrichter, Martin ; Synytska, Alla:
Conductive Hairy Particles With Homogeneous and Janus Design as Carrier Materials for the Efficient Immobilization of Unspecific Peroxygenases.
In: Biotechnology Journal. Bd. 20 (2025) Heft 7 . - e70078.
ISSN 1860-7314
DOI der Verlagsversion: https://doi.org/10.1002/biot.70078

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Abstract

Efficient immobilization of unspecific peroxygenases (UPOs) on hairy particles with homogeneous and Janus design possessing conductive core and polymeric shell was demonstrated. PDMAEMA brushes (hairs) with controlled chain lengths were successfully grown from the conductive silver particles allowing further immobilization of enzymes and keeping their activity. The Janus design of the synthesized particles maintained the conductivity of the core material. Enzyme immobilization on brush-modified particles was first carried out with two laccases as model enzymes Trametes versicolor (TveLac) and Pycnoporus cinnabarinus (PciLac) and then successfully extended to UPOs two wild-type and one recombinant UPO from Marasmius rotula (MroUPO) and Agrocybe aegerita (AaeUPO, rAaeUPO). The most efficient immobilization was achieved for MroUPO on Janus particles. The enzyme loading is reversible and could be successfully repeated after cleaning of the particles. Thus, the successful immobilization of an MroUPO on conductive hairy Janus particles was demonstrated for the first time. The major advantage of the proposed approach lies in the reusability of the enzyme and its carrier, as well as in the use of conductive core materials, which could be promising, for example, as material for electrochemical biosensors in future.