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Retracing the evolution of a modern periplasmic binding protein

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00007356
URN zum Zitieren der Version auf EPub Bayreuth: urn:nbn:de:bvb:703-epub-7356-4

Titelangaben

Michel, Florian ; Romero-Romero, Sergio ; Höcker, Birte:
Retracing the evolution of a modern periplasmic binding protein.
In: Protein Science. Bd. 32 (2023) Heft 11 . - e4793.
ISSN 1469-896X
DOI der Verlagsversion: https://doi.org/10.1002/pro.4793

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Abstract

Investigating the evolution of structural features in modern multidomain proteins helps to understand their immense diversity and functional versatility. The class of periplasmic binding proteins (PBPs) offers an opportunity to interrogate one of the main processes driving diversification: the duplication and fusion of protein sequences to generate new architectures. The symmetry of their two-lobed topology, their mechanism of binding, and the organization of their operon structure led to the hypothesis that PBPs arose through a duplication and fusion event of a single common ancestor. To investigate this claim, we set out to reverse the evolutionary process and recreate the structural equivalent of a single-lobed progenitor using ribose-binding protein (RBP) as our model. We found that this modern PBP can be deconstructed into its lobes, producing two proteins that represent possible progenitor halves. The isolated halves of RBP are well folded and monomeric proteins, albeit with a lower thermostability, and do not retain the original binding function. However, the two entities readily form a heterodimer in vitro and in-cell. The x-ray structure of the heterodimer closely resembles the parental protein. Moreover, the binding function is fully regained upon formation of the heterodimer with a ligand affinity similar to that observed in the modern RBP. This highlights how a duplication event could have given rise to a stable and functional PBP-like fold and provides insights into how more complex functional structures can evolve from simpler molecular components.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Keywords: flavodoxin-like fold; gene duplication; protein evolution; ribose binding protein; solute binding protein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie III - Proteindesign > Lehrstuhl Biochemie III - Proteindesign - Univ.-Prof. Dr. Birte Höcker
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie I - Proteinbiochemie der Signaltransduktion
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie III - Proteindesign
Sprache: Englisch
Titel an der UBT entstanden: Ja
URN: urn:nbn:de:bvb:703-epub-7356-4
Eingestellt am: 13 Dec 2023 08:23
Letzte Änderung: 13 Dec 2023 08:24
URI: https://epub.uni-bayreuth.de/id/eprint/7356

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