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URN to cite this document: urn:nbn:de:bvb:703-epub-7954-1
Title data
Toledo‐Patiño, Saacnicteh ; Goetz, Sara Kathrin ; Shanmugaratnam, Sooruban ; Höcker, Birte ; Farías‐Rico, José Arcadio:
Molecular handcraft of a well‐folded protein chimera.
In: FEBS Letters.
Vol. 598
(2024)
Issue 11
.
- pp. 1375-1386.
ISSN 1873-3468
DOI der Verlagsversion: https://doi.org/10.1002/1873-3468.14856
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Abstract
Modular assembly is a compelling pathway to create new proteins, a concept supported by protein engineering and millennia of evolution. Natural evolution provided a repository of building blocks, known as domains, which trace back to even shorter segments that underwent numerous 'copy-paste' processes culminating in the scaffolds we see today. Utilizing the subdomain-database Fuzzle, we constructed a fold-chimera by integrating a flavodoxin-like fragment into a periplasmic binding protein. This chimera is well-folded and a crystal structure reveals stable interfaces between the fragments. These findings demonstrate the adaptability of α/β-proteins and offer a stepping stone for optimization. By emphasizing the practicality of fragment databases, our work pioneers new pathways in protein engineering. Ultimately, the results substantiate the conjecture that periplasmic binding proteins originated from a flavodoxin-like ancestor.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | chimeric proteins; homology; protein engineering; protein fold evolution; sequence‐based analysis; sub‐domain |
| DDC Subjects: | 500 Science > 500 Natural sciences 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry III - Protein Design > Chair Biochemistry III - Protein Design - Univ.-Prof. Dr. Birte Höcker Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry III - Protein Design Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-7954-1 |
| Date Deposited: | 04 Oct 2024 07:07 |
| Last Modified: | 04 Oct 2024 07:07 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/7954 |
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