Impact of initial solvent on thermal stability and mechanical properties of recombinant spider silk films

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00004767
URN to cite this document: urn:nbn:de:bvb:703-epub-4767-0

Title data

Spiess, Kristina ; Ene, Roxana ; Keenan, Caroline D. ; Senker, Jürgen ; Kremer, Friedrich ; Scheibel, Thomas:
Impact of initial solvent on thermal stability and mechanical properties of recombinant spider silk films.
In: Journal of Materials Chemistry. Vol. 21 (2011) Issue 35 . - pp. 13594-13604.
ISSN 0959-9428
DOI der Verlagsversion: https://doi.org/10.1039/c1jm11700a

Format: PDF Name: c1jm11700a.pdf Version: Published Version Available under License German copyright law. This contribution is freely accessible with the consent of the copyright holder on the basis of an (DFG-funded) alliance or national license. The document may be used free of charge for personal use. In addition, the reproduction, editing, distribution and any kind of exploitation require the written consent of the respective rights holder.

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Abstract

Spider silk fibers are one of the most remarkable biopolymers displaying a unique combination of mechanical properties, biocompatibility and biodegradability. The recombinant production of spider silk proteins now allows the processing of silk proteins into novel materials with the aim of future applications. Here, we analyzed films made of a recombinantly produced, engineered spider silk protein with a sequence derived from the dragline silk protein ADF4 of the European garden spider Araneus diadematus. An influence of different initial solvents (aqueous buffer, hexafluoro-2-propanol and formic acid) on certain film properties was identified in as cast as well as methanol post-treated films: while no significant effects on the films’ thermal stability were observed, a significant influence on their mechanical properties could be shown. Interestingly, solvent-induced effects were sustained after methanol post-treatment and could be correlated with the presence and arrangement of secondary structure elements. Insights into molecular orientation of individual structural elements within the films upon applied load were revealed by combined polarized IR spectroscopy and mechanical measurements.

Further data

Item Type:	Article in a journal
Additional notes (visible to public):	ISI:000294176600063
DDC Subjects:	600 Technology, medicine, applied sciences > 620 Engineering
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Inorganic Chemistry III > Chair Inorganic Chemistry III - Univ.-Prof. Dr. Jürgen Senker Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Inorganic Chemistry III Faculties > Faculty of Engineering Science
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-4767-0
Date Deposited:	15 Sep 2020 08:53
Last Modified:	15 Sep 2020 08:53
URI:	https://epub.uni-bayreuth.de/id/eprint/4767

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