Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Natural Glycoforms of Human Interleukin 6 show atypical plasma clearance

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00005757
URN to cite this document: urn:nbn:de:bvb:703-epub-5757-0

Title data

Unverzagt, Carlo ; Reif, Andreas ; Lam, Kevin ; Weidler, Sascha ; Lott, Marie ; Boos, Irene ; Lokau, Juliane ; Bretscher, Christian ; Mönnich, Manuel ; Perkams, Lukas ; Schmälzlein, Marina ; Graf, Christopher ; Fischer, Jan-Patrick ; Lechner, Carolin ; Hallstein, Kerstin ; Becker, Stefan ; Weyand, Michael ; Steegborn, Clemens ; Schultheiss, Gerhard ; Rose-John, Stefan ; Garbers, Christoph:
Natural Glycoforms of Human Interleukin 6 show atypical plasma clearance.
In: Angewandte Chemie International Edition. Vol. 60 (2021) Issue 24 . - pp. 13380-13387.
ISSN 1521-3773
DOI der Verlagsversion: https://doi.org/10.1002/anie.202101496

[img]
Format: PDF
Name: anie.202101496.pdf
Version: Published Version
Available under License Creative Commons BY 4.0: Attribution
Download (2MB)

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract

A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis. The three segments were connected by sequential native chemical ligation followed by two-step refolding. The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans. Nine IL-6 glycoforms were synthesized, 7 of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli. Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan. The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses. Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.

Further data

Item Type: Article in a journal
Keywords: Glycoprotein; Native Chemical Ligation; Glycopeptide; Oliogosaccharide; Serum clearance
DDC Subjects: 500 Science > 540 Chemistry
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Bioorganic Chemistry > Chair Bioorganic Chemistry - Univ.-Prof. Dr. Carlo Unverzagt
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Bioorganic Chemistry
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-5757-0
Date Deposited: 03 Sep 2021 07:42
Last Modified: 03 Sep 2021 07:43
URI: https://epub.uni-bayreuth.de/id/eprint/5757

Downloads

Downloads per month over past year