in the repository
URN to cite this document: urn:nbn:de:bvb:703-epub-9084-1
Title data
Weyand, Michael ; Quast, Laura ; Steegborn, Clemens:
Potential 14-3-3 binding sites in sirtuins reveal extended phosphosite-recognition modes.
In: Acta Crystallographica Section F.
Vol. 82
(2026)
Issue 1
.
- pp. 32-40.
ISSN 2053-230X
DOI der Verlagsversion: https://doi.org/10.1107/S2053230X25010908
![[thumbnail of no5215.pdf]](https://epub.uni-bayreuth.de/style/images/fileicons/application_pdf.png) |
|
||||||||
|
Download (11MB)
|
Project information
| Project title: |
Project's official title Project's id Molekulare Mechanismen der physiologischen Regulation von Sirtuin 1 und Modulation dieser Mechanismen mit Wirkstoffen 277644855 Open Access Publizieren No information |
|---|---|
| Project financing: |
Deutsche Forschungsgemeinschaft |
Abstract
The adapter proteins of the 14-3-3 family modulate the activity and/or localization of their binding partners, which they capture if a generic target motif is in its phosphorylated state. Here, we report the identification of potential 14-3-3 binding sites in human sirtuin deacylases by bioinformatic analysis. We then characterize the interactions of peptides representing phosphorylation sites in sirtuin 3 (pS103) and sirtuin 1 (pS670) with 14-3-3 proteins. We further describe the crystal structures of complexes of 14-3-3σ with either of the two phosphopeptides. As a conclusion, we propose a more extended 14-3-3 binding mode on the N-terminal side of the phosphorylation site and the possibility of nongeneric motifs and conformations on the C-terminal side, still resulting in the known high binding affinity of the two partners.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | 14-3-3 proteins; sirtuins; phosphopeptides; regulation; protein-protein interaction |
| DDC Subjects: | 500 Science > 540 Chemistry |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-9084-1 |
| Date Deposited: | 07 Apr 2026 13:21 |
| Last Modified: | 07 Apr 2026 13:21 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/9084 |
Download Statistics