Chemoselective Attachment of the Water-Soluble Dark Quencher Hydrodabcyl to Amino Groups in Peptides and Preservation of Its Spectroscopic Properties over a Wide pH Range

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00006564
URN to cite this document: urn:nbn:de:bvb:703-epub-6564-4

Title data

Kempf, Oxana ; Ullmann, G. Matthias ; Schobert, Rainer ; Kempf, Karl ; Bombarda, Elisa:
Chemoselective Attachment of the Water-Soluble Dark Quencher Hydrodabcyl to Amino Groups in Peptides and Preservation of Its Spectroscopic Properties over a Wide pH Range.
In: ACS Omega. Vol. 6 (2021) Issue 48 . - pp. 32896-32903.
ISSN 2470-1343
DOI der Verlagsversion: https://doi.org/10.1021/acsomega.1c04891

	Format: PDF Name: acsomega.1c04891.pdf Version: Published Version Available under License Creative Commons BY-NC-ND 4.0: Attribution, Noncommercial, No Derivative Works
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Project information

Project title:	Project's official title Project's id SFB 1357 Mikroplastik 391977956 Eigene Stelle BO 3578/1 Open Access Publizieren No information
Project financing:	Deutsche Forschungsgemeinschaft Andere

Abstract

The water-soluble quencher hydrodabcyl can be activated as an N-succinimidyl ester that is readily accessible from crude hydrodabcyl and storable for a long time. With primary and secondary amines, it reacts swiftly and chemoselectively, even in the presence of other competing nucleophiles such as those typically present in natural peptides. One of the three phenolic OH groups of hydrodabcyl is amenable to selective mono-Boc protection resulting in reduced polarity, advantageous to its further use in organic synthesis. The advantages of hydrodabcyl over dabcyl in spectrometric applications are exemplified by the pH dependence of its absorbance spectra.

Further data

Item Type:	Article in a journal
DDC Subjects:	500 Science 500 Science > 530 Physics 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Organic Chemistry I - Photo- und Elektrokatalyse für Nachhaltigkeit Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Organic Chemistry I - Univ.-Prof. Dr. Rainer Schobert Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professor Bioinformatics/Structural Biology > Professor Bioinformatics/Structural Biology - Univ.-Prof. Dr. Matthias Ullmann Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professor Bioinformatics/Structural Biology Research Institutions Research Institutions > Collaborative Research Centers, Research Unit Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-6564-4
Date Deposited:	28 Jul 2022 10:14
Last Modified:	28 Jul 2022 10:14
URI:	https://epub.uni-bayreuth.de/id/eprint/6564

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