Interplay of Different Major Ampullate Spidroins during Assembly and Implications for Fiber Mechanics

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00005257
URN to cite this document: urn:nbn:de:bvb:703-epub-5257-4

Title data

Saric, Merisa ; Eisoldt, Lukas ; Döring, Volker ; Scheibel, Thomas:
Interplay of Different Major Ampullate Spidroins during Assembly and Implications for Fiber Mechanics.
In: Advanced Materials. Vol. 33 (2021) Issue 9 . - No. 2006499.
ISSN 1521-4095
DOI der Verlagsversion: https://doi.org/10.1002/adma.202006499

	Format: PDF Name: adma.202006499.pdf Version: Published Version Available under License Creative Commons BY-NC-ND 4.0: Attribution, Noncommercial, No Derivative Works
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Abstract

Major ampullate (MA) spider silk has fascinating mechanical properties combining strength and elasticity. All known natural MA silks contain at least two or more different spidroins; however, it is unknown why and if there is any interplay in the spinning dope. Here, two different spidroins from Araneus diadematus are co‐produced in Escherichia coli to study the possible dimerization and effects thereof on the mechanical properties of fibers. During the production of the two spidroins, a mixture of homo‐ and heterodimers is formed triggered by the carboxyl‐terminal domains. Interestingly, homodimeric species of the individual spidroins self‐assemble differently in comparison to heterodimers, and stoichiometric mixtures of homo‐ and heterodimers yield spidroin networks upon assembly with huge impact on fiber mechanics upon spinning. The obtained results provide the basis for man‐made tuning of spinning dopes to yield high‐performance fibers.

Further data

Item Type:	Article in a journal
Keywords:	Bioinspired fibers; Heterodimers; Protein interplay; Self‐assembly; Spider silk
DDC Subjects:	600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Profile Fields Profile Fields > Advanced Fields Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Emerging Fields Profile Fields > Emerging Fields > Food and Health Sciences Research Institutions Research Institutions > Central research institutes Research Institutions > Central research institutes > Bayreuth Center for Material Science and Engineering - BayMAT Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-5257-4
Date Deposited:	12 Feb 2021 08:14
Last Modified:	11 Jun 2021 09:05
URI:	https://epub.uni-bayreuth.de/id/eprint/5257

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