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Thermotoga maritima NusG : domain interaction mediates autoinhibition and thermostability

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Drögemüller, Johanna ; Schneider, Christin ; Schweimer, Kristian ; Strauß, Martin ; Wöhrl, Birgitta M. ; Rösch, Paul ; Knauer, Stefan H.:
Thermotoga maritima NusG : domain interaction mediates autoinhibition and thermostability.
In: Nucleic Acids Research. Vol. 45 (2017) Issue 1 . - pp. 446-460.
ISSN 1362-4962
DOI der Verlagsversion: https://doi.org/10.1093/nar/gkw1111

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Abstract

NusG, the only universally conserved transcription factor, comprises an N- and a C-terminal domain (NTD, CTD) that are flexibly connected and move in- dependently in Escherichia coli and other organisms. In NusG from the hyperthermophilic bacterium Thermotoga maritima (tmNusG), however, NTD and CTD interact tightly. This closed state stabilizes the CTD, but masks the binding sites for the interaction partners Rho, NusE and RNA polymerase (RNAP), suggesting that tmNusG is autoinhibited. Furthermore, tmNusG and some other bacterial NusGs have an additional domain, DII, of unknown function. Here we demonstrate that tmNusG is indeed autoinhibited and that binding to RNAP may stabilize the open conformation. We identified two interdomain salt bridges as well as Phe336 as major determinants of the domain interaction. By successive weakening of this interaction we show that after domain dissociation tmNusG-CTD can bind to Rho and NusE, similar to the Escherichia coli NusG-CTD, indicating that these interactions are conserved in bacteria. Furthermore, we show that tmNusG-DII interacts with RNAP as well as nucleic acids with a clear preference for double stranded DNA. We suggest that tmNusG-DII supports tmNusG recruitment to the transcription elongation complex and stabilizes the tmNusG:RNAP complex, a necessary adaptation to high temperatures.

Further data

Item Type: Article in a journal
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry IV - Biophysical Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Language: English
Originates at UBT: Yes
Date Deposited: 03 May 2017 07:05
Last Modified: 26 Jun 2020 10:46
URI: https://epub.uni-bayreuth.de/id/eprint/3660

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