Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Long-Read Amplicon Sequencing of Nitric Oxide Dismutase (nod) Genes Reveal Diverse Oxygenic Denitrifiers in Agricultural Soils and Lake Sediments

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00005109
URN to cite this document: urn:nbn:de:bvb:703-epub-5109-7

Title data

Zhu, Baoli ; Wang, Zhe ; Kanaparthi, Dheeraj ; Kublik, Susanne ; Ge, Tida ; Casper, Peter ; Schloter, Michael ; Lueders, Tillmann:
Long-Read Amplicon Sequencing of Nitric Oxide Dismutase (nod) Genes Reveal Diverse Oxygenic Denitrifiers in Agricultural Soils and Lake Sediments.
In: Microbial Ecology. Vol. 80 (1 July 2020) Issue 1 . - pp. 243-247.
ISSN 1432-184X
DOI der Verlagsversion: https://doi.org/10.1007/s00248-020-01482-0

[img]
Format: PDF
Name: Zhu2020_Article_Long-ReadAmpliconSequencingOfN.pdf
Version: Published Version
Available under License Creative Commons BY 4.0: Attribution
Download (766kB)

Abstract

Microorganisms play an essential role in nitrogen cycling and greenhouse gas emissions in soils and sediments. The recently discovered oxygenic denitrifiers are proposed to reduce nitrate and nitrite via nitric oxide dismutation directly to N2 and O2. So far, the ecological role of these microbes is not well understood. The only available tool for a targeted study of oxygenic denitrifiers is their respective maker gene, nitric oxide dismutase (nod). Here, we established the use of PacBio long-read sequencing of nod gene amplicons to study the diversity and community structure of oxygenic denitrifiers. Two distinct sets of environmental samples, agricultural soil and lake sediment, were investigated as examples. The circular consensus sequences (ca 1.0 kb) obtained covered most substitution characteristic of NO dismutase and allowed for reliable classification of oxygenic denitrifiers. Distinct nod gene pools and community structure were revealed for the different habitats, with most sequence types affiliated to yet unidentified environmental nod lineages. The abundance of nod genes ranged 2.2 × 106–3.2 × 107 gene copies g−1 soil or sediment, accounting for up to 3% of total bacterial 16S rRNA gene counts. This study indicates that nod-gene-targeted long-read sequencing can be a powerful tool for studying the ecology of these novel microbes, and the results also suggest that oxygenic denitrifiers are prevalent and abundant in different terrestrial samples, where they could play an important, but yet overlooked role in nitrogen transformations.

Further data

Item Type: Article in a journal
DDC Subjects: 500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology > Chair Ecological Microbiology - Univ.-Prof. Dr. Tillmann Lüders
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions
Research Institutions > Research Centres
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-5109-7
Date Deposited: 24 Sep 2020 10:41
Last Modified: 24 Sep 2020 10:42
URI: https://epub.uni-bayreuth.de/id/eprint/5109

Downloads

Downloads per month over past year