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SuhB is an integral part of the ribosomal antitermination complex and interacts with NusA

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00004637
URN to cite this document: urn:nbn:de:bvb:703-epub-4637-9

Title data

Dudenhöffer, Benjamin R. ; Schneider, Hans ; Schweimer, Kristian ; Knauer, Stefan H.:
SuhB is an integral part of the ribosomal antitermination complex and interacts with NusA.
In: Nucleic Acids Research. Vol. 47 (25 May 2019) Issue 12 . - pp. 6504-6518.
ISSN 1362-4962
DOI der Verlagsversion: https://doi.org/10.1093/nar/gkz442

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Abstract

The synthesis of ribosomal RNA (rRNA) is a tightly regulated central process in all cells. In bacteria efficient expression of all seven rRNA operons relies on the suppression of termination signals (antitermination) and the proper maturation of the synthesized rRNA. These processes depend on N-utilization substance (Nus) factors A, B, E and G, as well as ribosomal protein S4 and inositol monophosphatase SuhB, but their structural basis is only poorly understood. Combining nuclear magnetic resonance spectroscopy and biochemical approaches we show that Escherichia coli SuhB can be integrated into a Nus factor-, and optionally S4-, containing antitermination complex halted at a ribosomal antitermination signal. We further demonstrate that SuhB specifically binds to the acidic repeat 2 (AR2) domain of the multi-domain protein NusA, an interaction that may be involved in antitermination or posttranscriptional processes. Moreover, we show that SuhB interacts with RNA and weakly associates with RNA polymerase (RNAP). We finally present evidence that SuhB, the C-terminal domain of the RNAP α-subunit, and the N-terminal domain of NusG share binding sites on NusA-AR2 and that all three can release autoinhibition of NusA, indicating that NusA-AR2 serves as versatile recruitment platform for various factors in transcription regulation.

Further data

Item Type: Article in a journal
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-4637-9
Date Deposited: 27 Mar 2020 11:58
Last Modified: 27 Mar 2020 11:58
URI: https://epub.uni-bayreuth.de/id/eprint/4637

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