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A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle

URN to cite this document: urn:nbn:de:bvb:703-epub-4320-0

Title data

Raschdorf, Oliver ; Bonn, Florian ; Zeytuni, Natalie ; Zarivach, Raz ; Becher, Dörte ; Schüler, Dirk:
A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle.
Bayreuth, Germany , 2018 . - 45 S.

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Project information

Project financing: Deutsche Forschungsgemeinschaft (Schu1080/9-2 and 15-3)
ERC Advanced Grant No. 692637

Abstract

Magnetotactic bacteria produce chains of complex membrane-bound organelles that direct the biomineralization of magnetic nanoparticles and serve for magnetic field navigation. These magnetosome compartments have recently emerged as a model for studying the subcellular organization of prokaryotic organelles. Previous studies indicated the presence of specific proteins with various functions in magnetosome biosynthesis. However, the exact composition and stoichiometry of the magnetosome subproteome have remained unknown. In order to quantify and unambiguously identify all proteins specifically targeted tothe magnetosome membrane of the Alphaproteobacterium Magnetospirillum gryphiswaldense, we analyzed the protein composition of several cellular fractions by semi-quantitative mass spectrometry. We found that nearly all genuine magnetosome membrane-integral proteins belong to a well-defined set of previously identified proteins encoded by gene clusters within a genomic island, indicating a highly controlled protein composition. Magnetosome proteins were present in different quantities with up to 120 copies per particle as estimated by correlating our results with available quantitative Western blot data. This high abundance suggests an unusually crowded protein composition of the membrane and a tight packing with transmembrane domains of integral proteins. Our findings will help to further define the structure of the organelle and contribute to the elucidation of magnetosome biosynthesis.

Further data

Item Type: Preprint, postprint
Additional notes (visible to public): In: Journal of Proteomics, Bd. 172 (2018, S. 89-99 ISSN 1874-3919
This project has received funding from Deutsche Forschungsgemeinschaft (Schu1080/9-2 and 15-3) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 692637)
Keywords: Bacterial organelles; Magnetosomes; Membrane integral sub-proteome; Protein quantification
DDC Subjects: 500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-4320-0
Date Deposited: 25 Apr 2019 11:14
Last Modified: 03 May 2019 07:09
URI: https://epub.uni-bayreuth.de/id/eprint/4320

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