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Generation of nanomagnetic biocomposites by genetic engineering of bacterial magnetosomes

URN to cite this document: urn:nbn:de:bvb:703-epub-4314-6

Title data

Mickoleit, Frank ; Schüler, Dirk:
Generation of nanomagnetic biocomposites by genetic engineering of bacterial magnetosomes.
Bayreuth, Germany , 2019 . - 43 S.

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Project information

Project title:
Project's official titleProject's id
DFG Schwerpunktprogramm SPP 1569 "Generation of multifunctional inorganic materials by molecular bionics"DFG SPP Schu1080/15-1, 15-2 and 15-3
ERC AdG Syntomagx692637

Project financing: Deutsche Forschungsgemeinschaft
European Research Council (ERC)

Abstract

Magnetosomes are magnetic nanoparticles biomineralized by magnetotactic bacteria. They consist of a monocrystalline magnetite core enveloped by the magnetosome membrane, which harbors a set of specialized proteins. For the alphaproteobacterium Magnetospirillum gryphiswaldense genetic techniques were developed for engineering both crystal morphology and the enveloping membrane, thereby generating building blocks for magnetic organic–inorganic hybrid materials. Genetic manipulation of magnetite biomineralization enabled the generation of core-engineered nanoparticles with adjusted magnetic and physicochemical properties. Functionalization of the particle surface was achieved by genetic expression of enzymes and peptides genetically fused to abundant magnetosome anchor proteins. High-level expression allowed the generation of multifunctional nanoparticles with maximized protein-to-particle ratios. This allowed for the tuning of surface properties (charge and hydrodynamic diameter), and the colloidal and enzymatic stability was improved by coating with inorganic and organic shells. The expression of molecular connectors might serve as scaffolds for the introduction of further functionalities. Overall, this demonstrates that the ‘synthetic biology’ approach enables the generation of multifunctional, magnetic hybrid materials with a tuned property spectrum exceeding those of conventional materials, and the combination of different biogenic materials generates fully genetically encoded biocomposites with enhanced potential for various biotechnological and biomedical applications.

Further data

Item Type: Preprint, postprint
Additional notes (visible to public): In: Bioinspired, Biomimetic and Nanobiomaterials, Bd. 8 (2018) Heft1, S. 86-98, ISSN 2045-9858, E-ISSN 2045-9866
This project has received funding from the Deutsche Forschungsgemeinschaft (grants DFG SPP Schu1080/15-1, 15-2 and 15-3) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC AdG Syntomagx to D.S.; grant agreement No 692637).
Keywords: Nanobiomaterial; nanoparticles; functional nanomaterials; magnetic materials
DDC Subjects: 500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-4314-6
Date Deposited: 23 May 2019 08:32
Last Modified: 23 May 2019 08:32
URI: https://epub.uni-bayreuth.de/id/eprint/4314

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