Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Generation of multifunctional magnetic nanoparticles with amplified catalytic activities by genetic expression of enzyme arrays on bacterial magnetosomes

URN to cite this document: urn:nbn:de:bvb:703-epub-4311-0

Title data

Mickoleit, Frank ; Schüler, Dirk:
Generation of multifunctional magnetic nanoparticles with amplified catalytic activities by genetic expression of enzyme arrays on bacterial magnetosomes.
Bayreuth, Germany , 2017 . - 32 S.

[img] PDF
Manuscript F. Mickoleit.pdf - Accepted Version
Available under License Deutsches Urheberrechtsgesetz .

Download (5MB)

Related URLs

Project information

Project title:
Project's official titleProject's id
DFG Schwerpunktprogramm SPP 1569 "Generation of multifunctional inorganic materials by molecular bionics"Schu 1080/15-3
ERC AdG Syntomagx692637

Project financing: Deutsche Forschungsgemeinschaft
European Research Council (ERC) Advanced Grants

Abstract

Owing to their highly regulated biosynthesis, magnetosomes biomineralized by magnetotactic bacteria represent natural magnetic nanoparticles with unique physical and chemical properties. They consist of a magnetite core that is surrounded by a biological membrane, and are therefore reminiscent to magnetic “core-shell” nanoparticles. Their usability in many nanotechnological and biomedical applications would be further improved by the introduction of additional catalytic and imaging modalities. Here, a new in vivo strategy is explored for magnetosome display of foreign polypeptides with maximized protein-to-particle ratios. Arrays of up to five monomers of the model enzyme glucuronidase GusA plus the additional fluorophore mEGFP are genetically fused as single large hybrid proteins to highly expressed magnetosome protein anchors. In total, about 190 GusA monomers are covalently attached to individual particles. Assuming layers of GusA rows surrounding the particles, the monomers would thus cover up to 90% of the magnetosome surface. Our approach generates nanoparticles that exhibit magnetism, fluorescence and stable catalytic activities, which were step-wise increased with the number of GusA monomers. In summary, multi-copy expression of arrayed foreign proteins represents a powerful methodology for the biosynthesis of tailored biohybrid magnetic nanoparticles with several genetically encoded and tunable functionalities.

Further data

Item Type: Preprint, postprint
Additional notes (visible to public): In: Advanced Biosystems, Bd. 2 (2018) Heft 1. - No. 1700109. ISSN: 2366-7478
This project has received funding from the Deutsche Forschungsgemeinschaft (grant DFG SPP SCHU 1080/15-3) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC AdG Syntomagx to D.S.; grant agreement No 692637)
Keywords: bacterial nanoparticles; magnetosome expression; surface functionalization; glucuronidase; enzyme kinetics
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-4311-0
Date Deposited: 12 Apr 2019 09:31
Last Modified: 25 Apr 2019 10:53
URI: https://epub.uni-bayreuth.de/id/eprint/4311

Downloads

Downloads per month over past year