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Exploring RNA polymerase regulation by NMR spectroscopy

DOI zum Zitieren dieses Dokuments: https://doi.org/10.1038/srep10825

Title data

Drögemüller, Johanna ; Strauß, Martin ; Schweimer, Kristian ; Wöhrl, Birgitta M. ; Knauer, Stefan H. ; Rösch, Paul:
Exploring RNA polymerase regulation by NMR spectroscopy.
In: Scientific Reports. Vol. 5 (June 2015) . - Article number: 10825.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep10825

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Abstract

RNA synthesis is a central process in all organisms, with RNA polymerase (RNAP) as the key enzyme. Multisubunit RNAPs are evolutionary related and are tightly regulated by a multitude of transcription factors. Although Escherichia coli RNAP has been studied extensively, only little information is available about its dynamics and transient interactions. This information, however, are crucial for the complete understanding of transcription regulation in atomic detail. To study RNAP by NMR spectroscopy we developed a highly efficient procedure for the assembly of active RNAP from separately expressed subunits that allows specific labeling of the individual constituents. We recorded [¹H,¹³C] correlation spectra of isoleucine, leucine, and valine methyl groups of complete RNAP and the separately labeled β’ subunit within reconstituted RNAP. We further produced all RNAP subunits individually, established experiments to determine which RNAP subunit a certain regulator binds to, and identified the β subunit to bind NusE.

Further data

Item Type: Article in a journal
Keywords: Solution-state NMR; transcriptional regulatory elements
DDC Subjects: 500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry I
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Language: English
Originates at UBT: Yes
Date Deposited: 01 Oct 2015 08:09
Last Modified: 08 Feb 2017 10:44
URI: https://epub.uni-bayreuth.de/id/eprint/3120

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