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Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition

DOI zum Zitieren dieses Dokuments: https://doi.org/10.1371/journal.pone.0102444

Title data

Schmidt, Holger ; Günther, Carmen ; Weber, Michael ; Spörlein, Cornelia ; Loscher, Sebastian ; Böttcher, Christoph ; Schobert, Rainer ; Clemens, Stephan:
Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition.
In: PLOS ONE. Vol. 9 (2014) Issue 7 . - e102444.
ISSN 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0102444

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Abstract

Fe deficiency compromises both human health and plant productivity. Thus, it is important to understand plant Fe acquisition strategies for the development of crop plants which are more Fe-efficient under Fe-limited conditions, such as alkaline soils, and have higher Fe density in their edible tissues. Root secretion of phenolic compounds has long been hypothesized to be a component of the reduction strategy of Fe acquisition in non-graminaceous plants. We therefore subjected roots of Arabidopsis thaliana plants grown under Fe-replete and Fe-deplete conditions to comprehensive metabolome analysis by gas chromatography-mass spectrometry and ultra-pressure liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Scopoletin and other coumarins were found among the metabolites showing the strongest response to two different Fe-limited conditions, the cultivation in Fe-free medium and in medium with an alkaline pH. A coumarin biosynthesis mutant defective in ortho-hydroxylation of cinnamic acids was unable to grow on alkaline soil in the absence of Fe fertilization. Co-cultivation with wild-type plants partially rescued the Fe deficiency phenotype indicating a contribution of extracellular coumarins to Fe solubilization. Indeed, coumarins were detected in root exudates of wild-type plants. Direct infusion mass spectrometry as well as UV/vis spectroscopy indicated that coumarins are acting both as reductants of Fe(III) and as ligands of Fe(II).

Further data

Item Type: Article in a journal
Additional notes (visible to public): BAYCEER123856
Keywords: Coumarins; Iron deficiency; Arabidopsis thaliana; Metabolites; Plant biochemistry; Metabolomics; Gas chromatography-mass spectrometry; Phenols
DDC Subjects: 500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Genetics
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Language: English
Originates at UBT: Yes
Date Deposited: 01 Dec 2015 11:20
Last Modified: 26 Feb 2019 10:53
URI: https://epub.uni-bayreuth.de/id/eprint/2117

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