Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00009125
URN to cite this document: urn:nbn:de:bvb:703-epub-9125-9

Title data

Mohotti, Supun ; Mannala, Gopala K. ; Bargel, Hendrik ; Alt, Volker ; Scheibel, Thomas:
Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella.
In: Advanced NanoBiomed Research. Vol. 5 (2025) Issue 4 . - 2400160.
ISSN 2699-9307
DOI der Verlagsversion: https://doi.org/10.1002/anbr.202400160

	Format: PDF Name: Advanced NanoBiomed Research - 2025 - Mohotti - Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk.pdf Version: Published Version Available under License Creative Commons BY 4.0: Attribution
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Project information

Project title:	Project's official title Project's id TRR 225: Von den Grundlagen der Biofabrikation zu funktionalen Gewebemodellen 326998133 Open Access Publizieren No information
Project financing:	Deutsche Forschungsgemeinschaft

Abstract

The microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as Staphylococcus aureus. Utilizing Galleria mellonella as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing Galleria mellonella as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.

Further data

Item Type:	Article in a journal
Keywords:	bacterial repellences; dip coating; in vivo models; multidrug-resistant bacteria; spray coating; Staphylococcus aureus
DDC Subjects:	600 Technology, medicine, applied sciences > 620 Engineering
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Research Institutions > Central research institutes > Bayreuth Center for Colloids and Interfaces - BZKG Research Institutions > Central research institutes > Bayreuth Center for Molecular Biosciences - BZMB Research Institutions > Central research institutes > Bayreuth Center for Material Science and Engineering - BayMAT Research Institutions > Affiliated Institutes > Bavarian Polymer Institute (BPI) Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Research Institutions Research Institutions > Central research institutes Research Institutions > Affiliated Institutes
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-9125-9
Date Deposited:	16 Apr 2026 07:30
Last Modified:	16 Apr 2026 07:30
URI:	https://epub.uni-bayreuth.de/id/eprint/9125

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