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URN to cite this document: urn:nbn:de:bvb:703-epub-8842-8
Title data
Maneira, Carla ; Becker, Sina ; Chamas, Alexandre ; Lackner, Gerald:
A multilayered biocontainment system for laboratory and probiotic yeast.
In: Metabolic Engineering.
Vol. 91
(2025)
.
- pp. 442-454.
ISSN 1096-7184
DOI der Verlagsversion: https://doi.org/10.1016/j.ymben.2025.06.009
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Project information
| Project title: |
Project's official title Project's id EXC 2051: Gleichgewicht im Mikroversum 390713860 Open Access Publizieren No information |
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| Project financing: |
Deutsche Forschungsgemeinschaft |
Abstract
The containment of genetically engineered microorganisms to designated environments of action is a paramount step in preventing their spread to nature. Physical barriers were traditionally employed to solve this issue, nevertheless, the growing number of biotechnological operations in open dynamic environments calls for intrinsic biocontainment. Here we describe the development of genetically embedded safeguard systems for both a laboratory strain of Saccharomyces cerevisiae and the commercial probiotic Saccharomyces cerevisiae var. boulardii. In a stepwise approach, single-input metabolic circuits based either on a synthetic auxotrophy or a CRISPR-based kill switch were developed before their combination into an orthogonal two-input system. All circuits are based on gut-active molecules or environmental cues, making them amenable to microbiome therapy applications. The final two-input system is stable for more than a hundred generations while achieving less than one escapee in 109 CFUs after incubation under restrictive conditions for at least six days. Biocontained strains can robustly produce heterologous proteins under permissive conditions, supporting their future use in the most varied applications, like in-situ production and delivery of pharmaceutically active metabolites.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | biocontainment system; probiotic yeast; genetically engineered microorganisms; Saccharomyces cerevisia |
| DDC Subjects: | 500 Science > 500 Natural sciences 500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Institutions of the University: | Faculties > Faculty of Life Sciences: Food, Nutrition and Health > Chair Biochemistry of Microorganisms > Chair Biochemistry of Microorganisms - Univ.-Prof. Dr. Gerald Lackner Faculties Faculties > Faculty of Life Sciences: Food, Nutrition and Health Faculties > Faculty of Life Sciences: Food, Nutrition and Health > Chair Biochemistry of Microorganisms |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-8842-8 |
| Date Deposited: | 02 Feb 2026 13:41 |
| Last Modified: | 02 Feb 2026 13:42 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/8842 |
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