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URN to cite this document: urn:nbn:de:bvb:703-epub-8657-0
Title data
Ranzani, Américo Tavares ; Buchholz, Konrad ; Blackholm, Marius ; Kopkin, Hayat ; Möglich, Andreas:
Induction of bacterial expression at the mRNA level by light.
In: Nucleic Acids Research.
Vol. 52
(2024)
Issue 16
.
- pp. 10017-10028.
ISSN 1362-4962
DOI der Verlagsversion: https://doi.org/10.1093/nar/gkae678
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Project information
| Project title: |
Project's official title Project's id Photoaktivierte RNA-Bindung in einem Blaulicht-Rezeptor für optoribogenetische Anwendungen 315180630 Struktur, Funktion und Optoribogenetik RNA-bindender Blaulicht-Rezeptoren 522261769 Open Access Publizieren No information |
|---|---|
| Project financing: |
Deutsche Forschungsgemeinschaft |
Abstract
Vital organismal processes, including development, differentiation and adaptation, involve altered gene expression. Although expression is frequently controlled at the transcriptional stage, various regulation mechanisms operate at downstream levels. Here, we leverage the photoreceptor NmPAL to optogenetically induce RNA refolding and the translation of bacterial mRNAs. Blue-light-triggered NmPAL binding disrupts a cis-repressed mRNA state, thereby relieves obstruction of translation initiation, and upregulates gene expression. Iterative probing and optimization of the circuit, dubbed riboptoregulator, enhanced induction to 30-fold. Given action at the mRNA level, the riboptoregulator can differentially regulate individual structural genes within polycistronic operons. Moreover, it is orthogonal to and can be wed with other gene-regulatory circuits for nuanced and more stringent gene-expression control. We thus advance the pAurora2 circuit that combines transcriptional and translational mechanisms to optogenetically increase bacterial gene expression by >1000-fold. The riboptoregulator strategy stands to upgrade numerous regulatory circuits and widely applies to expression control in microbial biotechnology, synthetic biology and materials science.
Further data
| Item Type: | Article in a journal |
|---|---|
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry II - Photobiochemistry > Chair Biochemistry II - Photobiochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry II - Photobiochemistry |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-8657-0 |
| Date Deposited: | 26 Nov 2025 11:48 |
| Last Modified: | 26 Nov 2025 11:48 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/8657 |
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