Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00008394
URN to cite this document: urn:nbn:de:bvb:703-epub-8394-5

Title data

Mayer, Moritz ; Schug, Christina ; Geimer, Stefan ; Klecker, Till ; Westermann, Benedikt:
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy.
In: Microbial Cell. Vol. 11 (2024) Issue 1 . - pp. 378-386.
ISSN 2311-2638
DOI der Verlagsversion: https://doi.org/10.15698/mic2024.11.840

	Format: PDF Name: 2024a-mayer-microbial-cell.pdf Version: Published Version Available under License Creative Commons BY 4.0: Attribution
	Download (3MB)

Project information

Project title:	Project's official title Project's id FOR2092 239484859 Open Access Publizieren No information
Project financing:	Deutsche Forschungsgemeinschaft Elitenetzwerk Bayern

Abstract

Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM). Preparation of yeast cells for TEM can be quite challenging and time-consuming. Here, we describe an optimized protocol for conventional fixation of yeast cells with potassium permanganate combined with cell wall permeabilization with sodium metaperiodate and embedding in Epon. We have replaced time-consuming incubation steps by short treatments with microwaves and developed a microwave-assisted permanganate fixation and Epon embedding protocol that reduces the time required for sample preparation to one working day. We expect that these protocols will be useful for routine analysis of membrane ultrastructure in yeast.

Further data

Item Type:	Article in a journal
Keywords:	Saccharomyces cerevisiae; microwave fixation; mitochondria; organelle architecture; potassium permanganate fixation; transmission electron microscopy;
DDC Subjects:	500 Science > 570 Life sciences, biology
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology > Professor Cell Biology - Univ.-Prof. Dr. Benedikt Westermann Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-8394-5
Date Deposited:	02 Apr 2025 07:47
Last Modified:	02 Apr 2025 07:48
URI:	https://epub.uni-bayreuth.de/id/eprint/8394

Download Statistics

Download Statistics

Download Statistics

Downloads

Downloads per month over past year