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URN to cite this document: urn:nbn:de:bvb:703-epub-8380-2
Title data
Meier, Stefanie S. M. ; Multamäki, Elina ; Ranzani, Américo T. ; Takala, Heikki ; Möglich, Andreas:
Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling.
In: Nature Communications.
Vol. 15
(2024)
.
- 4876.
ISSN 2041-1723
DOI der Verlagsversion: https://doi.org/10.1038/s41467-024-49251-8
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Project's official title Project's id Open Access Publizieren No information |
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Deutsche Forschungsgemeinschaft |
Abstract
Bacteria must constantly probe their environment for rapid adaptation, a crucial need most frequently served by two-component systems (TCS). As one component, sensor histidine kinases (SHK) control the phosphorylation of the second component, the response regulator (RR). Downstream responses hinge on RR phosphorylation and can be highly stringent, acute, and sensitive because SHKs commonly exert both kinase and phosphatase activity. With a bacteriophytochrome TCS as a paradigm, we here interrogate how this catalytic duality underlies signal responses. Derivative systems exhibit tenfold higher red-light sensitivity, owing to an altered kinase-phosphatase balance. Modifications of the linker intervening the SHK sensor and catalytic entities likewise tilt this balance and provide TCSs with inverted output that increases under red light. These TCSs expand synthetic biology and showcase how deliberate perturbations of the kinase-phosphatase duality unlock altered signal-response regimes. Arguably, these aspects equally pertain to the engineering and the natural evolution of TCSs.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | Protein design; Expression systems; Synthetic biology; Kinases |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry II - Photobiochemistry > Chair Biochemistry II - Photobiochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry II - Photobiochemistry |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-8380-2 |
| Date Deposited: | 31 Mar 2025 07:32 |
| Last Modified: | 31 Mar 2025 07:33 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/8380 |
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