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URN to cite this document: urn:nbn:de:bvb:703-epub-7139-3
Title data
Ingino, Pablo ; Tiew, Kai Hao ; Obst, Martin:
Suitability of lectin binding studies for the characterization of redox-active microbial environmental biofilms.
In: AMB Express.
Vol. 12
(2022)
.
- No. 140.
ISSN 2191-0855
DOI der Verlagsversion: https://doi.org/10.1186/s13568-022-01479-7
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Project information
| Project title: |
Project's official title Project's id Open Access Publizieren No information No information OB 362/7-1 |
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| Project financing: |
Deutsche Forschungsgemeinschaft |
Abstract
Aquatic environmental microbial biofilms grow in a broad range of redox environments from oxic to methano- genic, and they often also establish internal redox gradients. In technical applications, biofilms are also subjected to controlled redox conditions. Studies on biofilms often make use of fluorescence microscopic imaging techniques together with lectin binding analysis to gain insights into structure, composition, and functions of the biofilms. Here we studied the direct influence of redox potentials on fluorescence lectin binding analyses (FLBA) for two commonly used lectin-fluorophore conjugates. An effect of the electrical potential on signal intensity was observed and found to be statistically significant. The signal intensity changes however, remained within the range of a few percent total. A significant drop in intensity was only observed for extremely oxidizing potentials, typically not found under envi- ronmental conditions. Our results showed that the fluorophore itself and not the lectin binding to the respective gly- coconjugate causes fluorescence changes. The two tested lectin-fluorophores are shown to be suitable for studying the distribution and composition of EPS in environmental biofilms or technical applications and under varying redox conditions.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | Biofilm; Redox potential; Glycoconjugates; Lectin; EPS; FLBA |
| DDC Subjects: | 500 Science > 550 Earth sciences, geology |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry > Heisenberg Professorship - Experimental Biogeochemistry - Univ.-Prof. Dr. Martin Obst Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-7139-3 |
| Date Deposited: | 21 Jul 2023 10:01 |
| Last Modified: | 18 Mar 2024 08:01 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/7139 |
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