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URN to cite this document: urn:nbn:de:bvb:703-epub-7120-5
Title data
Lamberger, Zan ; Bargel, Hendrik ; Humenik, Martin:
Aptamer-Modified Nanohydrogel Microarrays for Bioselective Cancer Cell Immobilization.
In: Advanced Functional Materials.
Vol. 32
(2022)
Issue 45
.
- No. 2207270.
ISSN 1616-3028
DOI der Verlagsversion: https://doi.org/10.1002/adfm.202207270
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Project information
| Project title: |
Project's official title Project's id BTHA Grant No. JC-2019-21 No information |
|---|---|
| Project financing: |
Bavarian-Czech Academic Agency |
Abstract
Abstract Photolithography combined with surface nucleated protein self-assembly of azido-modified spider silk proteins is used to create an arbitrarily shaped, inherently cell repellent micropattern based on nanofibrillar networks. Using “click” chemistry with dibenzocyclooctin modified oligonucleotides, the microstructures are functionalized with DNA-aptamers, which selectively bind cancer cell markers protein tyrosine kinase 7 or nucleolin. The epitope-specific cell interaction on the aptamer-modified surfaces is tested using human non-adherend leukemia T cells (Jurkat), as well as adherent cervix carcinoma (HeLa) and neuroblastoma (Kelly) cells. The cells can be immobilized with high precision and cell densities on the pattern, also revealing spatially defined proliferation and spreading into distinct morphologies upon cultivation. The formation of integrin-based focal adhesions occurs in the case of the aptamer immobilized cancer cells, similarly to those anchored on RGD-modified pattern. The firm aptamer-marker anchorage allows for the formation of integrin-dependent cell adhesions. Due to the amenability of the recombinant spider silk protein towards chemical and genetical modifications, the presented micropatterned fibrous networks have great potential for further development of adjustable and biocompatible cell-specific arrays, enabling applications in circulating cancer cell isolation and cultivation, studies on the cell's pathogenesis, progression and metastasis capabilities as well as enabling development of platforms for personalized medicine.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | aptamers; cancer cells; micropattern; nanofibrils; nanohydrogels; patterning; photolithography |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-7120-5 |
| Date Deposited: | 19 Jul 2023 06:37 |
| Last Modified: | 19 Jul 2023 06:38 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/7120 |
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