Cultivation of Encapsulated Primary Human B Lymphocytes : A First Step toward a Bioartificial Germinal Center

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00007102
URN to cite this document: urn:nbn:de:bvb:703-epub-7102-5

Title data

Helm, Moritz ; Huang, Songyan Ben ; Gollner, Katrin ; Gollner, Ulrich ; Jérôme, Valérie ; Freitag, Ruth:
Cultivation of Encapsulated Primary Human B Lymphocytes : A First Step toward a Bioartificial Germinal Center.
In: Macromolecular Bioscience. Vol. 23 (2023) Issue 1 . - No. 2200256.
ISSN 1616-5195
DOI der Verlagsversion: https://doi.org/10.1002/mabi.202200256

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Project information

Project financing:	Deutsche Forschungsgemeinschaft

Abstract

Polyelectrolyte microcapsules based on sodium cellulose sulfate (SCS) and poly-diallyl-dimethyl-ammonium chloride (PDADMAC) have previously been proposed as a suitable ex vivo microenvironment for the cultivation and differentiation of primary human T lymphocytes. Here, the same system is investigated for the cultivation of human primary B cells derived from adult tonsillar tissue. Proliferation and differentiation into subtypes are followed and compared to suspension cultures of B cells from the same pool performed in parallel. Total cell expansion is somewhat lower in the capsules than in the suspension cultures. More importantly, however, the differentiation of the initially mainly memory B cells into various subtypes, in particular into plasma cell (PC), shows significant differences. Clearly, the microenvironment provided by the microcapsules is beneficial for an accelerated induction of a germinal center-like B cell phenotype and afterward supports the long-term survival of the PC cells. Then, varying the encapsulation conditions (i.e., presence of human serum and dedicated cytokines in the capsule core) provides a tool for finetuning the B cell response. Hence, this methodology is suggested to pave the way toward ex vivo development of human immune organoids.

Further data

Item Type:	Article in a journal
Keywords:	biomimetic microenvironment; differentiation; encapsulation; germinal center; human primary B lymphocytes
DDC Subjects:	500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 600 Technology 600 Technology, medicine, applied sciences > 610 Medicine and health
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-7102-5
Date Deposited:	17 Jul 2023 06:42
Last Modified:	17 Jul 2023 06:43
URI:	https://epub.uni-bayreuth.de/id/eprint/7102

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