Transcriptional Response of Two Brassica napus Cultivars to Short-Term Hypoxia in the Root Zone

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00006900
URN to cite this document: urn:nbn:de:bvb:703-epub-6900-6

Title data

Ambros, Stefanie ; Kotewitsch, Mona ; Wittig, Philipp R. ; Bammer, Bettina ; Mustroph, Angelika:
Transcriptional Response of Two Brassica napus Cultivars to Short-Term Hypoxia in the Root Zone.
In: Frontiers in Plant Science. Vol. 13 (2022) .
ISSN 1664-462X
DOI der Verlagsversion: https://doi.org/10.3389/fpls.2022.897673

	Format: PDF Name: fpls-13-897673.pdf Version: Published Version Available under License Creative Commons BY 4.0: Attribution
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Project information

Project title:	Project's official title Project's id BayKlimaFit TGC01GCUFuE69742 Open Access Publizieren No information
Project financing:	Deutsche Forschungsgemeinschaft Bayerisches Staatsministerium für Umwelt und Verbraucherschutz

Abstract

Waterlogging is one major stress for crops and causes multiple problems for plants, for example low gas diffusion, changes in redox potential and accumulation of toxic metabolites. Brassica napus is an important oil crop with high waterlogging sensitivity, which may cause severe yield losses. Its reactions to the stress are not fully understood. In this work the transcriptional response of rapeseed to one aspect of waterlogging, hypoxia in the root zone, was analyzed by RNAseq, including two rapeseed cultivars from different origin, Avatar from Europe and Zhongshuang 9 from Asia. Both cultivars showed a high number of differentially expressed genes in roots after 4 and 24 h of hypoxia. The response included many well-known hypoxia-induced genes such as genes coding for glycolytic and fermentative enzymes, and strongly resembled the hypoxia response of the model organism Arabidopsis thaliana. The carbohydrate status of roots, however, was minimally affected by root hypoxia, with a tendency of carbohydrate accumulation rather than a carbon starvation. Leaves did not respond to the root stress after a 24-h treatment. In agreement with the gene expression data, subsequent experiments with soil waterlogging for up to 14 days revealed no differences in response or tolerance to waterlogging between the two genotypes used in this study. Interestingly, using a 0.1 starch solution for waterlogging, which caused a lowered soil redox potential, resulted in much stronger effects of the stress treatment than using pure water suggesting a new screening method for rapeseed cultivars in future experiments.

Further data

Item Type:	Article in a journal
Keywords:	Brassica napus; waterlogging; root-zone hypoxia; fermentation; RNA sequencing
DDC Subjects:	500 Science > 570 Life sciences, biology 500 Science > 580 Plants (Botany)
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-6900-6
Date Deposited:	21 Mar 2023 09:05
Last Modified:	21 Mar 2023 09:05
URI:	https://epub.uni-bayreuth.de/id/eprint/6900

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