Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00006652
URN to cite this document: urn:nbn:de:bvb:703-epub-6652-3

Title data

Gorbovytska, Vladyslava ; Kim, Seung-Kyoon ; Kuybu, Filiz ; Götze, Michael ; Um, Dahun ; Kang, Keunsoo ; Pittroff, Andreas ; Brennecke, Theresia ; Schneider, Lisa-Marie ; Leitner, Alexander ; Kim, Tae-Kyung ; Kuhn, Claus-D.:
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.
In: Nature Communications. Vol. 13 (2022) . - No. 2429.
ISSN 2041-1723
DOI der Verlagsversion: https://doi.org/10.1038/s41467-022-29934-w

	Format: PDF Name: s41467-022-29934-w.pdf Version: Published Version Available under License Creative Commons BY 4.0: Attribution
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Project information

Project title:	Project's official title Project's id Verdrängen eRNAs NELF von RNA polymerase und aktivieren damit die Transkription? No information Open Access Publizieren No information
Project financing:	Deutsche Forschungsgemeinschaft KU 3514/1-1 und KU 3514/3-1

Abstract

Enhancer RNAs (eRNAs) are long non-coding RNAs that originate from enhancers. Although eRNA transcription is a canonical feature of activated enhancers, the molecular features required for eRNA function and the mechanism of how eRNAs impinge on target gene transcription have not been established. Thus, using eRNA-dependent RNA polymerase II (Pol II) pause release as a model, we examined the requirement of sequence, structure and length of eRNAs for their ability to stimulate Pol II pause release by detaching NELF from paused Pol II. We found eRNA not to exert their function through common structural or sequence motifs. Instead, eRNAs that exhibit a length >200 nucleotides and that contain unpaired guanosines make multiple, allosteric contacts with NELF subunits -A and -E to trigger efficient NELF release. By revealing the molecular determinants of eRNA function, our study establishes eRNAs as an important player in Pol II pause release, and it provides new insight into the regulation of metazoan transcription.

Further data

Item Type:	Article in a journal
DDC Subjects:	500 Science > 570 Life sciences, biology
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry VI - RNA Biochemistry > Chair Biochemistry VI - RNA Biochemistry - Univ.-Prof. Dr. Claus-Dieter Kuhn Profile Fields > Advanced Fields > Molecular Biosciences Graduate Schools Graduate Schools > Bayreuth Graduate School of Mathematical and Natural Sciences (BayNAT) > Molecular Biosciences Graduate Schools > Elite Network Bavaria > Structural Basis of Gene Regulation by Non-coding RNA Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry VI - RNA Biochemistry Profile Fields Profile Fields > Advanced Fields Graduate Schools > Bayreuth Graduate School of Mathematical and Natural Sciences (BayNAT) Graduate Schools > Elite Network Bavaria
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-6652-3
Date Deposited:	15 Sep 2022 08:54
Last Modified:	18 Nov 2022 06:21
URI:	https://epub.uni-bayreuth.de/id/eprint/6652

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