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URN to cite this document: urn:nbn:de:bvb:703-epub-6484-9
Title data
Washburn, Robert S. ; Zuber, Philipp ; Sun, Ming ; Hashem, Yaser ; Shen, Bingxin ; Li, Wen ; Harvey, Sho ; Reyes, Francisco J. Acosta ; Gottesman, Max E. ; Knauer, Stefan H. ; Frank, Joachim:
Escherichia coli NusG links the lead ribosome with the transcription elongation complex.
In: iScience.
Vol. 23
(2020)
Issue 8
.
- No. 101352.
ISSN 2589-0042
DOI der Verlagsversion: https://doi.org/10.1016/j.isci.2020.101352
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Abstract
It has been known for more than 50 years that transcription and translation are physically coupled in bacteria, but whether or not this coupling may be mediated by the two-domain protein N-utilization substance (Nus) G in Escherichia coli is still heavily debated. Here, we combine integrative structural biology and functional analyses to provide conclusive evidence that NusG can physically link transcription with translation by contacting both RNA polymerase and the ribosome. We present a cryo-electron microscopy structure of a NusG:70S ribosome complex and nuclear magnetic resonance spectroscopy data revealing simultaneous binding of NusG to RNAP and the intact 70S ribosome, providing the first direct structural evidence for NusG-mediated coupling. Furthermore, in vivo reporter assays show that recruitment of NusG occurs late in transcription and strongly depends on translation. Thus, our data suggest that coupling occurs initially via direct RNAP:ribosome contacts and is then mediated by NusG.
Further data
| Item Type: | Article in a journal |
|---|---|
| DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry IV - Biophysical Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-6484-9 |
| Date Deposited: | 08 Jul 2022 10:26 |
| Last Modified: | 08 Jul 2022 10:26 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/6484 |
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