In vivo coating of bacterial magnetic nanoparticles by magnetosome expression of spider silk-inspired peptides

URN to cite this document: urn:nbn:de:bvb:703-epub-4312-5

Title data

Mickoleit, Frank ; Borkner, Christian B. ; Toro-Nahuelpan, Mauricio ; Herold, Heike M. ; Maier, Denis S. ; Plitzko, Jürgen M. ; Scheibel, Thomas ; Schüler, Dirk:
In vivo coating of bacterial magnetic nanoparticles by magnetosome expression of spider silk-inspired peptides.
Bayreuth , 2018 . - 41 S.

	Format: PDF Name: Manuscript spider silk coating F. Mickoleit.pdf Version: Accepted Version Available under License German copyright law. The document may be used free of charge for personal use. In addition, the reproduction, editing, distribution and any kind of exploitation require the written consent of the respective rights holder.
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	Format: PDF Name: Supporting Information.pdf Version: Accepted Version Available under License German copyright law. The document may be used free of charge for personal use. In addition, the reproduction, editing, distribution and any kind of exploitation require the written consent of the respective rights holder.
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Project information

Project title:	Project's official title Project's id DFG Schwerpunktprogramm SPP 1569 "Generation of multifunctional inorganic materials by molecular bionics" No information ERC AdG Syntomagx 692637
Project financing:	Deutsche Forschungsgemeinschaft European Research Council (ERC) Advanced Grants

Abstract

Magnetosomes are natural magnetic nanoparticles with exceptional properties that are synthesized in magnetotactic bacteria by a highly regulated biomineralization process. Their usability in many applications could be further improved by encapsulation in biocompatible polymers. In this study, we explored the production of spider silk-inspired peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion of different silk sequence-like variants to abundant magnetosome membrane proteins enhanced magnetite biomineralization and caused the formation of a proteinaceous capsule, which increased the colloidal stability of isolated particles. Furthermore, we show that spider silk peptides fused to a magnetosome membrane protein can be used as seeds for silk fibril growth on the magnetosome surface. In summary, we demonstrate that the combination of two different biogenic materials generates a genetically encoded hybrid composite with engineerable new properties and enhanced potential for various applications.

Further data

Item Type:	Preprint, postprint
Additional notes (visible to public):	In: Biomacromolecules, Bd. 19 (2018) Heft 3, S. 962–972. ISSN: 1526-4602 This project has received funding from the Deutsche Forschungsgemeinschaft (grants DFG SPP SCHU 1080/15-3 and SCHU 1080/9-2 to D.S., SCHE 603/15-2 to T.S.) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC AdG Syntomagx to D.S.; grant agreement No 692637)
Keywords:	Magnetosprillum gryphiswaldense; magnetosomes; surface functionalization; spider silk; biocomposite; hybrid proteins
DDC Subjects:	500 Science > 500 Natural sciences 500 Science > 570 Life sciences, biology
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Research Institutions > Central research institutes > Bayreuth Center for Molecular Biosciences - BZMB Research Institutions > Central research institutes > Research Center for Bio-Macromolecules - BIOmac Faculties Research Institutions Research Institutions > Central research institutes
Language:	English
Originates at UBT:	Yes
URN:	urn:nbn:de:bvb:703-epub-4312-5
Date Deposited:	12 Apr 2019 09:19
Last Modified:	25 Apr 2019 10:33
URI:	https://epub.uni-bayreuth.de/id/eprint/4312

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