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Title data
Riedl, Simon ; Kaiser, Patrick ; Raup, Alexander ; Synatschke, Christopher V. ; Jérôme, Valérie ; Freitag, Ruth:
Non-Viral Transfection of Human T Lymphocytes.
In: Processes.
Vol. 6
(2018)
Issue 10
.
- No. 188.
ISSN 2227-9717
DOI der Verlagsversion: https://doi.org/10.3390/pr6100188
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Abstract
The genetic modification of human T lymphocytes with established non-viral methods is inefficient. Linear polyethylenimine (l-PEI), one of the most popular non-viral transfection agents for mammalian cells in general, only achieves transfection rates in the single digit percentage range for these cells. Here, a well-defined 24-armed poly(2-dimethylamino) ethyl methacrylate (PDMAEMA) nanostar (number average of the molecular weight: 755 kDa, polydispersity: <1.21) synthesized via atom transfer radical polymerization (ATRP) from a silsesquioxane initiator core is proposed as alternative. The agent is used to prepare polyplexes with plasmid DNA (pDNA). Under optimal conditions these polyplexes reproducibly transfect >80% of the cells from a human T-cell leukemia cell line (Jurkat cells) at viabilities close to 90%. The agent also promotes pDNA uptake when simply added to a mixture of cells and pDNA. This constitutes a particular promising approach for efficient transient transfection at large scale. Finally, preliminary experiments were carried out with primary T cells from two different donors. Results were again significantly better than for l-PEI, although further research into the response of individual T cells to the transfection agent will be necessary, before either method can be used to routinely transfect primary T lymphocytes.
Further data
| Item Type: | Article in a journal |
|---|---|
| Keywords: | non-viral gene delivery; Jurkat cells; human T lymphocytes; primary cells;
poly(2-dimethylamino) ethyl methacrylate; polycation; nanostar |
| DDC Subjects: | 500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 600 Technology 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science |
| Language: | English |
| Originates at UBT: | Yes |
| URN: | urn:nbn:de:bvb:703-epub-4253-7 |
| Date Deposited: | 01 Mar 2019 12:19 |
| Last Modified: | 22 Sep 2023 11:36 |
| URI: | https://epub.uni-bayreuth.de/id/eprint/4253 |
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