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Escherichia coli NusG links the lead ribosome with the transcription elongation complex

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00006484
URN to cite this document: urn:nbn:de:bvb:703-epub-6484-9

Title data

Washburn, Robert S. ; Zuber, Philipp ; Sun, Ming ; Hashem, Yaser ; Shen, Bingxin ; Li, Wen ; Harvey, Sho ; Reyes, Francisco J. Acosta ; Gottesman, Max E. ; Knauer, Stefan H. ; Frank, Joachim:
Escherichia coli NusG links the lead ribosome with the transcription elongation complex.
In: iScience. Vol. 23 (2020) Issue 8 . - No. 101352.
ISSN 2589-0042
DOI der Verlagsversion: https://doi.org/10.1016/j.isci.2020.101352

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Abstract

It has been known for more than 50 years that transcription and translation are physically coupled in bacteria, but whether or not this coupling may be mediated by the two-domain protein N-utilization substance (Nus) G in Escherichia coli is still heavily debated. Here, we combine integrative structural biology and functional analyses to provide conclusive evidence that NusG can physically link transcription with translation by contacting both RNA polymerase and the ribosome. We present a cryo-electron microscopy structure of a NusG:70S ribosome complex and nuclear magnetic resonance spectroscopy data revealing simultaneous binding of NusG to RNAP and the intact 70S ribosome, providing the first direct structural evidence for NusG-mediated coupling. Furthermore, in vivo reporter assays show that recruitment of NusG occurs late in transcription and strongly depends on translation. Thus, our data suggest that coupling occurs initially via direct RNAP:ribosome contacts and is then mediated by NusG.

Further data

Item Type: Article in a journal
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-6484-9
Date Deposited: 08 Jul 2022 10:26
Last Modified: 08 Jul 2022 10:26
URI: https://epub.uni-bayreuth.de/id/eprint/6484

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