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In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00006043
URN to cite this document: urn:nbn:de:bvb:703-epub-6043-1

Title data

Riedl, Simon ; Völkl, Matthias ; Holzinger, Anja ; Jasinski, Julia ; Jérôme, Valérie ; Scheibel, Thomas ; Feldhaar, Heike ; Freitag, Ruth:
In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies.
In: Ecotoxicology. Vol. 31 (2021) . - pp. 221-233.
ISSN 1573-3017
DOI der Verlagsversion: https://doi.org/10.1007/s10646-021-02495-2

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Project information

Project title:
Project's official titleProject's id
SFB 1357 MikroplastikA02/A05

Project financing: Deutsche Forschungsgemeinschaft

Abstract

The earthworm Eisenia fetida is a commonly used model organism for unspecific soil feeders in ecotoxicological studies. Its intestinal cells are the first to encounter possible pollutants co-ingested by the earthworm, which makes them prime candidates for studies of toxic effects of environmental pollutants on the cellular as compared to the organismic level. Here, cells are isolated directly from the intestine, maintaining > 90% viability during subsequent short-time cultivations (up to 144 h). Exposure to established toxins comprising silver nanoparticles and metal ions (Cu2+, Cd2+) induced a significant decrease in the metabolic activity of the cells. In presence of microplastic particles (MP particles), namely 0.2, 0.5, 2.0, and 3.0 µm diameter polystyrene (PS) beads as well as 0.5 and 2.0 µm diameter polylactic acid (PLA) beads, no active uptake and no effect on the metabolic activity of the cells was observed. This suggests a tissue rather than cell related basis for the previously observed ecotoxicological effects of MP in case of Eisenia fetida.

Further data

Item Type: Article in a journal
Keywords: Earthworm; Eisenia fetida; Environmental pollutants; Microplastic; Cytotoxicity; Primary cells
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Biomaterials
Research Institutions
Research Institutions > Collaborative Research Centers, Research Unit
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-6043-1
Date Deposited: 15 Mar 2022 08:33
Last Modified: 15 Mar 2022 08:33
URI: https://epub.uni-bayreuth.de/id/eprint/6043

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