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Towards a "chassis" for bacterial magnetosome biosynthesis : genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions

DOI zum Zitieren der Version auf EPub Bayreuth: https://doi.org/10.15495/EPub_UBT_00005853
URN to cite this document: urn:nbn:de:bvb:703-epub-5853-3

Title data

Zwiener, Theresa ; Dziuba, Marina ; Mickoleit, Frank ; Rückert, Christian ; Busche, Tobias ; Kalinowski, Jörn ; Uebe, René ; Schüler, Dirk:
Towards a "chassis" for bacterial magnetosome biosynthesis : genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions.
In: Microbial Cell Factories. Vol. 20 (2021) Issue 1 . - No. 35.
ISSN 1475-2859
DOI der Verlagsversion: https://doi.org/10.1186/s12934-021-01517-2

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Project financing: Bundesministerium für Bildung und Forschung

Abstract

Abstract Background: Because of its tractability and straightforward cultivation, the magnetic bacterium Magnetospirillum gryphiswaldense has emerged as a model for the analysis of magnetosome biosynthesis and bioproduction. However, its future use as platform for synthetic biology and biotechnology will require methods for large-scale genome editing and streamlining. Results: We established an approach for combinatory genome reduction and generated a library of strains in which up to 16 regions including large gene clusters, mobile genetic elements and phage-related genes were sequentially removed, equivalent to ~ 227.6 kb and nearly 5.5% of the genome. Finally, the fragmented genomic magnetosome island was replaced by a compact cassette comprising all key magnetosome biosynthetic gene clusters. The prospective ’chassis’ revealed wild type-like cell growth and magnetosome biosynthesis under optimal conditions, as well as slightly improved resilience and increased genetic stability. Conclusion: We provide first proof-of-principle for the feasibility of multiple genome reduction and large-scale engineering of magnetotactic bacteria. The library of deletions will be valuable for turning M. gryphiswaldense into a microbial cell factory for synthetic biology and production of magnetic nanoparticles.

Further data

Item Type: Article in a journal
Keywords: Magnetospirillum gryphiswaldense; Magnetotactic bacteria; Magnetosomes; Genome reduction; Chassis; IS elements
DDC Subjects: 500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-5853-3
Date Deposited: 22 Oct 2021 08:51
Last Modified: 22 Oct 2021 08:52
URI: https://epub.uni-bayreuth.de/id/eprint/5853

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