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In vivo coating of bacterial magnetic nanoparticles by magnetosome expression of spider silk-inspired peptides

URN to cite this document: urn:nbn:de:bvb:703-epub-4312-5

Title data

Mickoleit, Frank ; Borkner, Christian B. ; Toro-Nahuelpan, Mauricio ; Herold, Heike M. ; Maier, Denis S. ; Plitzko, Jürgen M. ; Scheibel, Thomas ; Schüler, Dirk:
In vivo coating of bacterial magnetic nanoparticles by magnetosome expression of spider silk-inspired peptides.
Bayreuth , 2018 . - 41 S.

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Manuscript spider silk coating F. Mickoleit.pdf - Accepted Version
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Project information

Project title:
Project's official titleProject's id
DFG Schwerpunktprogramm SPP 1569 "Generation of multifunctional inorganic materials by molecular bionics"No information
ERC AdG Syntomagx692637

Project financing: Deutsche Forschungsgemeinschaft
European Research Council (ERC) Advanced Grants

Abstract

Magnetosomes are natural magnetic nanoparticles with exceptional properties that are synthesized in magnetotactic bacteria by a highly regulated biomineralization process. Their usability in many applications could be further improved by encapsulation in biocompatible polymers. In this study, we explored the production of spider silk-inspired peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion of different silk sequence-like variants to abundant magnetosome membrane proteins enhanced magnetite biomineralization and caused the formation of a proteinaceous capsule, which increased the colloidal stability of isolated particles. Furthermore, we show that spider silk peptides fused to a magnetosome membrane protein can be used as seeds for silk fibril growth on the magnetosome surface. In summary, we demonstrate that the combination of two different biogenic materials generates a genetically encoded hybrid composite with engineerable new properties and enhanced potential for various applications.

Further data

Item Type: Preprint, postprint
Additional notes (visible to public): In: Biomacromolecules, Bd. 19 (2018) Heft 3, S. 962–972. ISSN: 1526-4602
This project has received funding from the Deutsche Forschungsgemeinschaft (grants DFG SPP SCHU 1080/15-3 and SCHU 1080/9-2 to D.S., SCHE 603/15-2 to T.S.) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC AdG Syntomagx to D.S.; grant
agreement No 692637)
Keywords: Magnetosprillum gryphiswaldense; magnetosomes; surface functionalization; spider silk; biocomposite; hybrid proteins
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB
Research Institutions > Research Centres > Research Center for Bio-Macromolecules - BIOmac
Faculties
Research Institutions
Research Institutions > Research Centres
Language: English
Originates at UBT: Yes
URN: urn:nbn:de:bvb:703-epub-4312-5
Date Deposited: 12 Apr 2019 09:19
Last Modified: 25 Apr 2019 10:33
URI: https://epub.uni-bayreuth.de/id/eprint/4312

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