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Promoter, transgene, and cell line effects in the transfection of mammalian cells using PDMAEMA-based nano-stars

DOI zum Zitieren dieses Dokuments: https://doi.org/10.1016/j.btre.2016.05.003

Title data

Raup, Alexander ; Jérôme, Valérie ; Freitag, Ruth ; Synatschke, Christopher V. ; Müller, Axel H. E.:
Promoter, transgene, and cell line effects in the transfection of mammalian cells using PDMAEMA-based nano-stars.
In: Biotechnology Reports. Vol. 11 (June 2016) . - pp. 53-61.
ISSN 2215-017X
DOI: https://doi.org/10.1016/j.btre.2016.05.003

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Project financing: Andere
Oberfrankenstiftung,Bayreuth,Germany

Abstract

Non-viral transfection protocols are typically optimized using standard cells and reporter proteins, potentially underestimating cellular or transgene effects. Here such effects were studied for two human (Jurkat, HEK-293) and two rodent (CHO-K1, L929) cell lines and three fluorescent reporter proteins. Expression of the enhanced green fluorescent protein (EGFP) was studied under the control of the human elongation factor 1 alpha promoter and three viral promoters (SV40, SV40/enhancer, CMV), that of ZsYellow1 (yellow fluorescence) and mCherry (red fluorescence) for the CMV promoter. Results varied with the cell line, in particular for the Jurkat cells. Pair-wise co-transfection of the CMV controlled transgenes resulted in a significant fraction of monochromatic cells (EGFP for EGFP/YFP and EGFP/RFP co-transfections, YFP in case of YFP/RFP co-transfections). Only Jurkat cells were almost incapable of expressing YFP. Dilution of the plasmid DNA with a non-expressed plasmid showed cell line dependent effects on transfection efficiency and/or expression levels.

Further data

Item Type: Article in a journal
Keywords: Fluorescent reporter protein; Co-expression; Non-viral gene delivery; Mammalian cell; Poly(2-dimethylamino) ethyl methacrylate
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Language: English
Originates at UBT: Yes
Date Deposited: 05 Jul 2016 06:39
Last Modified: 19 Apr 2018 10:32
URI: https://epub.uni-bayreuth.de/id/eprint/3634

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