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Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast

DOI zum Zitieren dieses Dokuments: https://doi.org/10.1038/srep18344

Title data

Klecker, Till ; Wemmer, Megan ; Haag, Mathias ; Weig, Alfons ; Böckler, Stefan ; Langer, Thomas ; Nunnari, Jodi ; Westermann, Benedikt:
Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast.
In: Scientific Reports. Vol. 5 (2015) . - Article Number 18344.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep18344

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Project financing: Deutsche Forschungsgemeinschaft

Abstract

Membrane homeostasis affects mitochondrial dynamics, morphology, and function. Here we report genetic and proteomic data that reveal multiple interactions of Mdm33, a protein essential for normal mitochondrial structure, with components of phospholipid metabolism and mitochondrial inner membrane homeostasis. We screened for suppressors of MDM33 overexpression-induced growth arrest and isolated binding partners by immunoprecipitation of cross-linked cell extracts. These approaches revealed genetic and proteomic interactions of Mdm33 with prohibitins, Phb1 and Phb2, which are key components of mitochondrial inner membrane homeostasis. Lipid profiling by mass spectrometry of mitochondria isolated from Mdm33-overexpressing cells revealed that high levels of Mdm33 affect the levels of phosphatidylethanolamine and cardiolipin, the two key inner membrane phospholipids. Furthermore, we show that cells lacking Mdm33 show strongly decreased mitochondrial fission activity indicating that Mdm33 is critical for mitochondrial membrane dynamics. Our data suggest that MDM33 functionally interacts with components important for inner membrane homeostasis and thereby supports mitochondrial division.

Further data

Item Type: Article in a journal
Additional notes (visible to public): BAYCEER133211
DDC Subjects: 500 Science > 570 Life sciences, biology
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Cell Biology > Professorship Cell Biology - Univ.-Prof. Dr. Benedikt Westermann
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Cell Biology
Language: English
Originates at UBT: Yes
Date Deposited: 08 Jun 2016 09:07
Last Modified: 08 Feb 2017 10:44
URI: https://epub.uni-bayreuth.de/id/eprint/3167

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